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. 2016 Sep 2;7(51):84556–84574. doi: 10.18632/oncotarget.11821

Figure 7. Activation of integrin-mediated downstream signaling pathways in SW480 as compared to SW480/tria cells.

Figure 7

A. Activating phosphorylation of Erk (T202/Y204), S6 (S240/244) and Src (Y416) as well as expression of the integrin downstream GTPases Rac and RhoA in SW480 and SW480/tria cells treated with either triapine and cilengitide alone or in combination at the indicated concentrations were determined by Western blot. β-actin and vinculin were used as loading controls. B and C. SW480 and SW480/tria cells were treated with the indicated concentrations of dasatinib (Src inhibitor) alone (B) and in combination with triapine (C). After 72 h treatment, cell viability was determined by MTT assay. The values given are means and SD of one representative experiment out of three performed in triplicate. Statistical analysis was performed by two-way ANOVA (***P < 0.001). D. Combination indices (CI) for the 72 h analyses shown in C were calculated using CalcuSyn software. CI < 0.9, CI = 0.9 - 1.1 or CI > 1.1 represent synergism, additive effects and antagonism, respectively.