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. 2016 Sep 20;7(51):84575–84586. doi: 10.18632/oncotarget.12144

Figure 2. Relationship between SHP1 and NS5A in in vitro model of HCV infection.

Figure 2

A. SHP1 expression levels in Huh7.5 J6/JFH1 HCV infected cells. mRNA expression level and WB analyses of SHP1, 72 hrs post-infection. The results derived from 5 independent experiments. Data are expressed as mean ± S.D, **p<0.01. B. SHP1 interfering efficacy in both Huh7.5 J6/JFH1 and JFH1 A4 replicon system, after 72 hours of infection: mRNA expression levels, WB and relative densitomentric analysis of SHP1 after its silencing. Data are expressed as mean values ± S.D, **p<0.01. Beta-actin is used as a loading control. Immunoblot is representative of five independent experiments. C. mRNA expression levels, WB assay and relative densitomentric analysis of NS5A in Huh7.5 J6/JFH1 cells and JFH1 A4 replicon system, in presence or not of siSHP1. Beta-actin is used as a loading control. Immunoblot is representative of five independent experiments (**p<0.01).