Figure 1. GSI-I combined with ABT-737 reduces cell viability and induces apoptosis in melanoma cells, but not normal melanocytes in monolayer culture conditions.

(A) MTS assays of six melanoma cell lines and two human primary melanocyte cultures post indicated treatments. The viability of the DMSO control for each cell line was set to 100%. The combination significantly (p < 0.05 or less) reduced cell viability compared with DMSO or with single drug treated conditions in all melanoma cell lines. The statistical information was not added as it will make the figure difficult to read. (B) Bright field analysis of the experiment in Figure 1A. Scale bar = 100 μm. (C) The Annexin V assay of seven melanoma cell lines and one human primary melanocyte culture post indicated treatments. (D) Protein lysates were prepared under the same treatment conditions as above and were probed with an antibody recognizing full length and cleaved PARP. * indicates p < 0.05; ** indicates p < 0.01; *** indicates p < 0.001. All treatment time were for 48 hours.