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. 2016 Oct 13;7(51):84785–84797. doi: 10.18632/oncotarget.12626

Figure 5. Deletion of P1(-683) to P3(-393) up-regulated the luciferase activity in both HEC-1A and HEC-1B cells, and knocking down MZF1 up-regulated PAX2 transcription and expression in cells containing the repressive transcriptional region of the PAX2 promoter.

Figure 5

A. Deletion analysis of the PAX2 promoter in HEC-1A cells. B. Deletion analysis of the PAX2 promoter in HEC-1B cells. C. MZF1 mRNA was down-regulated in HEC-1B cells 24 to 48 hours after transfection of MZF1-siRNA. D. Luciferase reporter gene activity was increased after knocking down MZF1 in HEC-1B cells transfected with pGL3-P1, but not up-regulated in cells transfected with pGL3-P3, which did not contain the repressive transcriptional region of PAX2 promoter (-683 bp to -393 bp). E. PAX2 protein was up-regulated in HEC-1B cells 72 hours after MZF1-siRNA transfection. F. By analyzing the RNAseq data from TCGA database, an negative correlation was demonstrated between the expression of MZF1 and PAX2 (R=-0.263, p=0.043). *p<0.05.