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. 2016 Oct 19;7(51):84893–84906. doi: 10.18632/oncotarget.12753

Figure 5. MAZ transcriptional activates ZNF217 expression.

Figure 5

A. Six putative MAZ binding sites (MT1-MT6) are underlined in the promoter of ZNF217 promoter region. +1 indicates the first nucleotide upstream of ghe transcription start site (TSS). B. Transcriptional activity of ZNF217 was determined by luciferase assay under MAZ overexpression. PC3 cells was transfected with pGL3-ZNF217 promoter (WT, MT1 mutation, MT2 mutation, MT3 mutation, MT4 mutation, MT5 mutation, MT6 mutation) when cells were transfected with MAZ plasmids. C. ChIP assay was performed to detect the six binding sites in ZNF217 promoter region using anti-MAZ antibody or normal IgG. D, H. The binding ability of MAZ on the promoter of ZNF217 was assessed upon MAZ knockdown in PC3 cells or overexpression in LNCaP cells. E, I. The mRNA expression of MAZ, ZNF217 and FPN were examined MAZ knockdown in PC3 cells or overexpression in LNCaP cells. F, G, J, K. The cell growth and intracellular iron content were determined in MAZ knockdown PC3 cells or MAZ-transfected LNCaP cells. Data depict the mean ± SD and representative of six independent experiments. Asterisk (*) indicates P < 0.05