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. 2016 Oct 22;7(51):84907–84923. doi: 10.18632/oncotarget.12823

Figure 1. Expression and distribution of MKP-1 in the developing testes and LPS-induced acute testis inflammation.

Figure 1

Detection of MKP-1 expression in the developing testes (day 7 to day 60) was performed by realtime PCR A. and western blot B-C. analyses. Paraffin section of the testes of day 7 D., day 14 E., day 21 F., day 30 G. and day 60 H. were immunostained with anti-MKP-1 antibody. MKP-1 protein was detected in the Sertoli cells (red arrows), germ cells (blue arrows), some interstitial cells and blood vessel walls (black arrows). The Expression of Mkp-1 in mRNA J. and protein K, L. levels during LPS-induced acute testis inflammation. Histological analyses showed MKP-1 expression by LPS stimuli N and O. compared with control M. In addition, positive staining at nuclei and perinuclear region of cytoplasm of Sertoli cells were detected at Sertoli cell (red arrows) and TJs (blue arrows) after LPS treatments (m1-m3 vs. n1, o1-o2). I: IgG control. Scale bar: 50 μm (D to I, M to O) and 20 μm (d to h, m1 to m3, n1, o1 and o2). * p<0.05.