Figure 2. FITC fluorescence tracer assays to evaluate BTB permeability by LPS stimuli.

FITC fluorescence (green) was observed only in the basal area of seminiferous tubules of the control mice A., whereas in the mice during LPS-induced acute testis inflammation (5 day, B.) the tracer was also found in the adluminal compartment of seminiferous tubules. A1-A2, B1-B2: A magnified view of the boxed area in A and B, respectively. C and D. Same bright field images in A and B respectively were observed. Bars=100 μm (A - D) and 20 μm (A1, A2, B1 and B2).