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. 2016 Oct 22;7(51):84907–84923. doi: 10.18632/oncotarget.12823

Figure 4. Expression of MKP-1 and related signaling molecules in primary Sertoli cells by LPS stimuli.

Figure 4

A. Primary Sertoli cells were either not stimulated or stimulated with LPS (100 ng/ml). Cells were harvested for protein after 0, 5, 10, 15, 30, 60, 90, 120 and 240 minutes, and cell lysates were subjected to western blot analysis for MKP-1, p-ERK1/2, ERK2, p-JNK1/2, p-p38, p38 and β-actin. B. Primary Sertoli cells were either not stimulated or treated with LPS (1, 10, 100 and 1000 ng/ml) for 60 minutes. Cells were harvested and cell lysates were subjected to western blot analysis for MKP-1 and β-actin. Immunoblots were scanned, and the intensity of bands was quantified by the image program. Data were normalized to the control and expressed as the percentage of maximum activation of stimulation (a1-a4) or the ratio was expressed as fold change with respect to the control sample (b1). C. Primary Sertoli cells were either not stimulated or treated with LPS (100 ng/ml) for 60, 120 and 240 minutes. Cells were harvested for RNA, and realtime PCR was carried out using primers specific to Mkp-1 and β-actin. * p<0.05.