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. 2016 Oct 22;7(51):84907–84923. doi: 10.18632/oncotarget.12823

Figure 5. Expression of MKP-1 in TM4 Sertoli cells by LPS stimuli.

Figure 5

A. TM4 Sertoli cells were either not stimulated or stimulated with LPS (100 ng/ml). Cells were harvested for proteins after 0, 5, 10, 15, 30, 60, 90, 120, 240 and 360 minutes, and cell lysates were subjected to western blot analysis for MKP-1 and β-actin. B. TM4 Sertoli cells were either not stimulated or treated with LPS (1, 10, 100 and 1000 ng/ml) for 15 and 60 minutes. Cells were harvested and cell lysates were subjected to western blot analysis for MKP-1 and β-actin. Immunoblots were scanned, the intensity of bands was quantified by the image program. Data were normalized to the control, and the ratio was expressed as fold change in relation to the control sample C and D. E. TM4 Sertoli cells were either not stimulated or stimulated with LPS (100 ng/ml) for 1, 2, 4, 6, 8, 12, 24, 36 and 48 hours. Cells were harvested for RNA, and realtime PCR was carried out using primers specific to Mkp-1 and β-actin. F. TM4 Sertoli cells were either not stimulated or treated with LPS (1, 10, 100 and 1000 ng/ml) for 60 minutes. Cells were harvested for RNA, and realtime PCR was performed using primers specific to Mkp-1 and β-actin. * p<0.05.