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. 2016 Nov 4;7(51):84999–85020. doi: 10.18632/oncotarget.13113

Figure 2. PTEN is directly involved in the regulation of glucose uptake.

Figure 2

A. Cells were incubated for 1 hour with the fluorescent glucose analog probe 2-NBDG 50 μM, then the images were acquired immediately by fluorescence microscopy. Scale bar = 20 μM; Magnification = 63X. B. ImageJ quantification of the glucose uptake (average fluorescence intensity per cell) in the various cell lines. Five randomly chosen fields per sample were evaluated for a minimum of hundred cells. Data represented as mean + S.E.M. of four separate experiments. C. The cell lysates were analyzed by Western blotting to reveal the expression level of PTEN. A similar pattern of protein expression was reproduced in three other experiments. D. Representative agarose gel electrophoresis of PTEN specific mRNA obtained by RT-PCR amplification. GAPDH was used as house-keeping control and co-amplified in the same PCR reaction. E. Quantitation of PTEN mRNA and protein levels normalized to the respective house-keeping control.