Figure 6.

The pol3 and cdc27 mutations suppress dna2-C2. (A) The pol3-R18 mutation suppresses the temperature-sensitivity of dna2-C2. Cells from the indicated strains were spotted in 10-fold dilutions (10⁵, 10⁴, 10³, 10² and 10¹ cells) onto YE plates and incubated for 3 days at 36°C, 33°C or 30°C, or at 18°C for 6 days. (B) The cdc27-R22 mutation suppresses the temperature-sensitivity of dna2-C2 and the expression of wild-type cdc27⁺ abolishes the suppression. The dna2-C2 cdc27-R22 double mutant cells was transformed with pREP3X alone or pREP3X-cdc27 expressing wild-type cdc27⁺. Each transformant was grown in EMM containing thiamine (5 μg/ml) and the cells obtained were spotted in 10-fold dilutions (10⁵, 10⁴, 10³, 10² and 10¹ cells) onto EMM plates or EMM plate containing thiamine (5 μg/ml). The plates were incubated for 3 days at 36°C or 30°C.