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. 2016 Nov 8;7(51):85079–85096. doi: 10.18632/oncotarget.13196

Figure 2. IL-37b inhibits HCC cell growth and colony formation in vitro.

Figure 2

A. The growth of HCC SMMC-7721 and HepG2 cells with IL-37b overexpression or vector control was determined as described in Materials and Methods. ***P < 0.001. B. The colony formation assay was performed as described in Materials and Methods. The number of colonies was counted and compared. C. The cell cycle distribution of SMMC-7721 and HepG2 cells infected with LV_IL1F7b or LV_NC were analyzed as described in Materials and Methods. D. The protein expression levels of IL-37, cdc2, p21, c-myc, cyclin B1, cyclin A2, cyclin D1 were estimated in SMMC-7721 and HepG2 cells with IL-37b overexpression or vector control by Western blot analysis. β-actin was used as an internal control. The data shown are representative of three independent experiments with similar results. Data are expressed as mean±SD; Student's t test; nsp> 0.05; *p< 0.05, **p< 0.01, and ***p< 0.001.