Figure 3.

Repair of 3-meT lesions by hABH2 catalysed oxidative demethylation. The 3-meT containing oligonucleotide was incubated in the absence (solid line) or presence (dashed line) of hABH2, then degraded enzymatically to its constituent nucleosides by treatment with nuclease P1 and calf intestine alkaline phosphatase, and, finally, subjected to reverse-phase HPLC. The identity of the peaks corresponding to 2′-deoxycytidine (dC), 2′-deoxyguanosine (dG), 2′-deoxythymidine (dT), 2′-deoxyadenosine (dA), and 3-methyl-2′-deoxythymidine (3mdT) was inferred from comparison of their elution times with those of the corresponding reference standards.