Figure 2. Efficacy of OBP-401 against lymph-node metastasis of high-metastatic MDA-MB-231-RFP.

OBP-401 was injected intratumorally (1 × 10⁸ PFU) when the mice already developed metastasis in lymphatic ducts. A. Representative whole-body time-course images of mock-infected orthotopic tumors. B. Representative whole-body time-course images of orthotopic tumors after injection of OBP-401. C. Representative whole-body images of mock-infected and OBP-401-treated mice 6 weeks after tumor implantation. D. Comparison of lymphatic metastasis of mock-infected and OBP-401-treated mice. Fluorescent area and fluorescence intensity of lymph-node metastasis are calculated with ImageJ software. Data are shown as average ± SD. N = 7.