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. 2016 Nov 11;7(51):85291–85305. doi: 10.18632/oncotarget.13300

Figure 2. Cisplatin and dabigatran etexilate co-treatment reduces platelet activation and the generation of tissue factor-positive microparticles (TF+ MPs).

Figure 2

Upon sacrifice, blood was collected from ID8 tumor bearing mice, or non-tumor bearing mice (NTB), and 50 μl was treated with ammonium oxalate to lyse red blood cells for platelet analysis. The remaining whole blood was sequentially spun to remove all cells, generating platelet poor plasma (PPP). A. Non-tumor bearing mice were given either a single dose of dabigatran etexilate (80 mg/kg) by oral gavage or placed on dabigatran chow (10 mg/g) for 48 h. After tail transaction, total blood loss over 10 minutes was quantified. B. Activated platelets were identified by flow cytometry. C. TF+ MPs in PPP were quantified by flow cytometry. D. Cell origin of TF+ MPs stained with anti-CD41 (platelets), anti-CD45 (leukocytes), anti-CD31 (endothelial cells) and anti-CD326 (epithelial cells) antibodies in PPP isolated from ID8 tumor bearing mice. n = 5-10 mice per group. * = p<0.05 and # = p<0.01 compared to control vehicle-treated tumor bearing mice.