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. 2016 Nov 11;7(51):85291–85305. doi: 10.18632/oncotarget.13300

Figure 6. ID8 conditioned media (CM) promotes M2 polarization of IL-4 treated macrophages.

Figure 6

ID8 CM was collected after culture of ID8 cells for 48 hours in starving medium ± 0.1 Units/mL thrombin and ± 10 uM dabigatran. After centrifugation, ID8 CM was assayed for A. MCP-1 and B. VEGF. Murine RAW264.7 macrophage cells were treated with IL-4 in the presence or absence MCP-1 (3.2 ng/mL), thrombin (0.1 Units/mL) or ID8 conditioned media (10% by volume), and after 18 hours cell lysates were assayed for C. arginase activity (urea production). All cell cultures were assayed in at least quadruplicate. * = p<0.001 compared to indicated groups.