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. 2016 Nov 16;7(51):85381–85392. doi: 10.18632/oncotarget.13377

Figure 3. Pin1 depletion inhibits MAPK phosphorylation and cell migration.

Figure 3

A. Effect of siPin1 on p38, JNK, and ERK1/2 phosphorylation. hDPCs were transfected with Pin1-specific siRNA for 48 h, and the protein levels of p-p38, p-JNK, p-ERK1/2, and Pin1 were assessed. B, C. Effect of siPin1 on cell migration. The cells were transfected with siPin1 for 48 h. Migration assays were performed as described in the Materials and Methods section. The results represent the averages from three independent experiments. D, E. Effect of the Pin1 inhibitor PBi on ADP-induced migration. hDPCs were stimulated with ADP (100 μM) for 24 h in the absence or presence of 10 μM PBi. The graphs show the quantitative evaluation of the migration rates. F. hDPCs were treated with ADP and/or PBi for 24 h, and the protein levels of P2Y1 and Pin1 were assessed by Western blot analysis.