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. 2016 Nov 16;7(51):85381–85392. doi: 10.18632/oncotarget.13377

Figure 4. Loss of Pin1 accelerates proteasome-mediated degradation of P2Y1.

Figure 4

A. hDPCs were treated with 25 or 50 μM MG132 with or without thermal stress for 24 h, and a Western blot analysis for P2Y1 and Pin1 was performed. B. The cells were transfected with TAP-Pin1 for 24 h, subjected to thermal stress and and incubated for another 24 h. A Western blot analysis was performed to assess the P2Y1 levels. C. Effect of Pin1 on heat-treated cell lines stably expressing Pin1. MEF WT, MEF Pin1-/-, JB6-GFP, and JB6-Pin1 cells were subjected to thermal stress for 30 min, and the P2Y1 and Pin1 protein levels at the indicated recovery time points were detected by Western blot analysis. D, E. Pin1 stabilizes P2Y1. MEF Pin1-/- and JB6-GFP cells were transfected with 0.1 or 1 μg TAP-Pin1 plasmids for 48 h, and a Western blot analysis was performed to assess the P2Y1 protein levels.