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. 2016 Nov 16;7(51):85411–85429. doi: 10.18632/oncotarget.13392

Figure 7. p116-ERBB2 isoform is signaling impaired and degraded in lysosomes.

Figure 7

SKBR3 cells were untreated or treated with 20μM GA, 200nM BafilomycinA1 (Baf) alone or in combination for 2 hours at 37°C. The total p185-ERBB2, the GA-induced p116-ERBB2, p-AKT and TfR protein levels were assessed by Western blot using Abs recognizing the extracellular domain of ERBB2 (Ab-20), p-AKT and TfR. Calnexin was used as loading control. On the left side of the blot the migration of protein molecular mass standards expressed in kDa is shown.