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. 2016 Nov 16;7(51):85430–85436. doi: 10.18632/oncotarget.13397

Figure 3. Spiking Experiment on 3 healthy volunteers to determine sensitivity of DigPCR assay.

Figure 3

5 tubes of blood were obtained and drawn into Cell-Free DNA BCT tubes (Streck, Inc.). Appropriate serial dilutions of BRAF V600E DNA fragments were made and each tube was spiked with 1, 5, 25, or 125 copies/ml blood of BRAF V600E DNA. One tube was left un-spiked to serve as the negative control. In 2 of 3 samples, we could detect 1 molecule/ml of blood. We detected 5 molecules of BRAF V600E DNA fragments per mL of blood in all 3 samples.