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. 2016 Nov 16;7(51):85450–85463. doi: 10.18632/oncotarget.13398

Figure 1. Radiation induces NF-κB activity in Huh7/NF-κB-tk-luc2/rfp cells.

Figure 1

A. Huh7/NF-κB-tk-luc2/rfp cells were irradiated with 0–10 Gy and the BLI was performed to assess the NF-κB activity assay 48 h postirradiation. Radiation enhanced NF-κB activity in a dose-dependent manner. Significant elevation of NF-κB activity was found when cells received irradiation higher than 6 Gy. B. The IκBαM vector was used as a positive control for NF-κB inhibition and was transfected into the Huh7/NF-κB-tk-luc2/rfp cells. The quantified results showed that endogenous NF-κB activity was strikingly repressed by transfection of IκBαM vector. C. Higher cytotoxicity caused by radiation was observed in the cells transfected with the IκBαM vector as compared with nontransfected cells. D. Radiation induced the expressions of NF-κB downstream proteins were suppressed by transfected with the IκBαM vector. (*p< 0.05, **p< 0.01, as compared with that of the control).