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. 2016 Nov 16;7(51):85450–85463. doi: 10.18632/oncotarget.13398

Figure 4. Sorafenib inhibits the radiation-induced activity of NF-κB and its downstream proteins expressions via downregulating the ERK pathway.

Figure 4

A. Huh7/NF-κB-tk-luc2/rfp cells treated with 20 μM sorafenib, 50 μM PD98059 and transfected with the IκBαM vector were irradiated with or without 6 Gy radiation. Sorafenib, PD98059, and the IκBαM vector significantly suppressed NF-κB activity induced by radiation. B. EMSA was utilized to determine the DNA binding activity, which was suppressed by the combination therapy in all three HCC cell lines. C. The combination therapy reduced the expressions of NF-κB-regulated proteins induced by radiation. D. The combination therapy caused cell death through upregulating the expressions of apoptosis-related proteins. (*p< 0.05, **p< 0.01 as compared with the control group; #p < 0.05, ##p < 0.01 as compared with the RT group).