Figure 6. NF-κB activity and its downstream proteins are suppressed by pretreatment of sorafenib plus radiation. A. The NF-κB activity was assessed by the expression level of tk represented by the uptake of ¹³¹I-FIAU, which was decreased most significantly in the pretreatment of sorafenib plus RT group.

B. NF-κB/DNA binding activity was evaluated with EMSA, and NF-κB activity was strongly elevated by radiation and suppressed in the pretreatment of sorafenib plus RT group. C. The expressions of NF-κB downstream proteins were detected by Western blotting, and substantial decreases were found in the group pretreated with sorafenib plus RT.