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. 2004 Dec 1;32(21):6276–6283. doi: 10.1093/nar/gkh966

Figure 4.

Figure 4

Oligonucleotide-based dot-blot analysis of the sequence between tRNAE and COB. Oligonucleotides complementary to the non-coding region of the primary transcript were dotted onto a nylon filter and hybridized to labeled mitochondrial run-on transcripts. (A) Hybridization pattern obtained with labeled run-on transcripts from strain S150-Bio1 carrying a wild-type mitochondrial genome (ρ+). (B) Hybridization pattern obtained with labeled run-on transcripts from strain S150M9410 harboring a 910 bp deletion (Δ) in the intergenic and 5′-UTR region (ρM9410). The relative lengths of the non-coding regions in both strains as well as the position of the oligonucleotides are indicated below each autoradiogram.