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. 2016 Dec 19;8(2):2053–2068. doi: 10.18632/oncotarget.14026

Figure 1. Human squamous cell carcinoma can be xenotransplanted into humanized mice autologously.

Figure 1

a. Schematics of autologous reconstitution of HNSCC. b. After engraftment with CD34+ bone marrow cells from HNSCC patient undergoing surgery, humanized mice showed presence of human lymphocytic and myeloid cells (CD11b+), including quantifiable human NK cells (NKG2D+NKp46+) in the spleen. Gated mononuclear cells were plotted for human CD45 and murine CD45 levels (upper panels). Gated human CD45 then were plotted for human CD markers as noted in the axis (lower panels). In brief, peripheral blood was stained with murine CD45, human CD45, and c. Surgical tumor specimens from the same patient whose CD34+ BMC were used to engraft NOG-A2 mice (from 1b) were implanted into the same humanized mice. The engrafted tumor was harvested and stained for tumor infiltrating human CD8, CD4, and CD11b cells. The primary antibodies for these stainings were rabbit anti-human CD8, rat anti-human CD4, and rabbit anti-human CD11b conjugated to Alexa Fluor® 568. The bar scale is 100µ. Upper left panel is an H&E stain of the same tumor.