Table 2. Base–base helical parameters.
| Helical parameter | CGCUGCG GCGACGC | CGCFGCG GCGACGC | CGCPGCG GCGACGC | CGCFGCG GCGFCGC | CGUUACG GCAAUGC | CGUFACG GCAAUGC |
|---|---|---|---|---|---|---|
| <shear> | ||||||
| −1 | 0.0 (0.4) | 0.2 (0.5) | 0.1 (0.5) | 0.0 (0.4) | −0.2 (0.4) | 0.2 (0.4) |
| 0 | 0.1 (0.4) | −1.4 (2.7) | −1.2 (2.3) | 0.0 (2.4) | −0.1 (0.5) | 2.7 (2.5) |
| +1 | 0.0 (0.4) | 0.0 (0.4) | 0.1 (0.9) | −0.1 (0.4) | 0.0 (0.5) | 0.2 (0.6) |
| <stretch> | ||||||
| −1 | 0.3 (0.4) | 0.3 (0.4) | 0.2 (0.4) | 0.2 (0.3) | 0.2 (0.5) | 0.2 (0.3) |
| 0 | 0.3 (0.4) | 0.6 (1.0) | −0.9 (1.2) | −0.7 (1.1) | 0.4 (0.6) | −0.1 (1.0) |
| +1 | 0.3 (0.4) | 0.2 (0.3) | 0.5 (0.7) | 0.3 (0.3) | 0.4 (0.7) | 0.1 (0.7) |
| <stagger> | ||||||
| −1 | 0.0 (0.5) | −0.2 (0.4) | −0.3 (0.4) | −0.2 (0.5) | −0.1 (0.5) | −0.3 (0.5) |
| 0 | 0.0 (0.5) | −0.5 (1.0) | −0.6 (1.2) | −0.65 (1.0) | −0.1 (0.5) | 0.0 (1.1) |
| +1 | 0.0 (0.4) | −0.2 (0.4) | −0.4 (0.4) | −0.3 (0.4) | −0.1 (0.5) | −0.2 (0.7) |
| <buckle> | ||||||
| −1 | 2.0 (10) | 4.4 (11) | 13 (13) | 6.9 (11) | −0.7 (14) | −2.7 (11) |
| 0 | −0.6 (9) | −2.0 (11) | 15 (17) | −1.1 (14) | 3.2 (13) | −0.6 (13) |
| +1 | −6.0 (10) | −5.2 (10) | 3 (15) | −7.2 (11) | −1.2 (14) | −1.2 (12) |
| <propeller> | ||||||
| −1 | −16 (11) | −12 (8) | −8 (8) | −10 (9) | −11 (16) | −13 (10) |
| 0 | −16 (10) | −11 (11) | −10 (12) | −10 (11) | −14 (16) | −8 (13) |
| +1 | −17 (11) | −11 (8) | −3 (14) | −11 (8) | −14 (16) | −11 (11) |
| <opening> | ||||||
| −1 | 2.3 (5) | 3.4 (5) | 0.3 (5) | 1.0 (7) | 1.0 (6) | 3.2 (7) |
| 0 | 2.6 (6) | 14.8 (18) | −12 (26) | −7.0 (25) | 4.3 (6) | 2.4 (16) |
| +1 | 2.5 (5) | 1.8 (4) | 4 (9) | −2.1 (0) | 5.5 (6) | 6.7 (9) |
Average inter-base helical parameters (SDs in parenthesis) were calculated for the central base pair (position 0) and the 5′ (−1) and 3′ (+1) neighbouring base pairs (relevant sequence in bold, second strand in 3′ → 5′ direction) averaged over the entire data gathering period (2–5.1 ns) using Curves (29).