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. 2016 Aug 8;8(2):2197–2208. doi: 10.18632/oncotarget.11116

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Copyright: © 2017 Wu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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A. HEK293T cells were co-transfected with Flag-HDAC1 and Myc-USP19. Cell lysates were subjected to immunoprecipitated (IP) with anti-Flag, and immunoprecipitants or the whole-cell lysates (WCL) were analyzed by immunoblotting (IB) with anti-Myc and anti-Flag. B. HEK293T cells were cotransfected with Flag-HDAC2 and Myc-USP19. Cell lysates were subjected to immunoprecipitated with anti-Flag, and immunoprecipitants or the WCL were analyzed by immunoblotting with anti-Myc and anti-Flag. C. HCT116 cells were cotransfected as indicated. Two hours after irradiated with 5Gy γ-ray, cell lysates were immunoprecipitated by using Myc antibody, and the bound proteins were analyzed with Flag antibody. D. Two hours after irradiated with 5Gy γ-ray, HCT116 cell lysates were immunoprecipitated by using USP19 antibody, and the bound proteins were analyzed with HDAC1/2 antibody.