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PAGE showing stages in the purification of C.AhdI. Lane 1, molecular weight marker; lane 2, whole cell lysate; lane 3, ammonium sulfate pellet following resuspension and dialysis; lane 4, pooled heparin fractions; lane 5, resuspended protein precipitate; lane 6, pooled cation exchange fractions; lane 7, pooled size exclusion fractions; and lane 8, concentrated C.AhdI.
