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. 2016 Dec 1;8(2):2659–2671. doi: 10.18632/oncotarget.13736

Figure 1. Identification of cell subsets producing IL-10 after therapeutic vaccination.

Figure 1

Naive Vert-X mice (A–B), mice bearing 5 mm B16-OVA (C–D), TC-1 (E) or E.G7-OVA tumors (F) (n = 8–11/group) were vaccinated with antigen (OVA in A–D and F or EDA-HPV-E7 in E) plus Imiquimod, antigen plus poly(I:C) or left untreated (UT). Two days later spleens or lymph nodes were obtained and the percentage of IL-10-producing cells was determined by flow cytometry in total cells and in the different subsets. Results correspond to the sum of 2–3 independent experiments.