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. 2016 Dec 1;8(2):2745–2757. doi: 10.18632/oncotarget.13740

Figure 5. ALG-2 is important for the motility of breast cancer cells.

Figure 5

(A) Wound healing assays using MDA-MB-231 and BT549 cells transfected with control or ALG-2 siRNAs. Wound margins were imaged 16 hours after wounding. (B) Experiments were performed as in A, and the percentage of wound closure was quantified. (C) Wound healing assays using 4T1-luc cells transfected with control or mALG-2 siRNAs. (D) Experiments were performed as in C, and the percentage of wound closure was quantified. (E) Wound healing assays using BT549 cells transfected with RFP-V or RFP-ALG-2. (F) Experiments were performed as in E, and the percentage of wound closure was quantified. (G) Transwell migration assays using MDA-MB-231 and BT549 cells transfected with control or ALG-2 siRNAs. (H) Experiments were performed as in G, and the extent of transwell migration was quantified. (I) Transwell migration assays using 4T1-luc cells transfected with control or mALG-2 siRNAs. (J) Experiments were performed as in I, and the extent of transwell migration was quantified. (K–M) Analysis of random migration of MDA-MB-231 cells. Cell movement paths were tracked (K), and the velocity of cell movement (L) and the accumulated distance were analyzed (M). Error bars indicate means ± SEM. *p < 0.05; **p < 0.01; ns, not significant.