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. 2016 Dec 1;8(2):2874–2889. doi: 10.18632/oncotarget.13760

Figure 1. Met and Prop effect on breast tumor cells viability.

Figure 1

Cells were cultured in the presence of the indicated doses of Met (A) or Prop (B) during 24 hours. The number of metabolically active cells was estimated by tetrazolium salts reduction method (n = 3). (C) 4T1 (left panel), MDA-MB-231 (middle panel) and M-406-derived cells (right panel) were treated for 24 hours with Met (M; 1 mM), Prop (P; 1 μM) or a combination of them (M+P) and living cells were estimated as before (n = 3). (D–G) Cell proliferation and doubling time evaluation by real time impedance-based method for 4T1 (D, E) and MDA-MB-231 (F, G). Arrows indicate the moment of treatment addition (Met 1 mM, Prop 10 μM).