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. 2016 Dec 1;8(2):2874–2889. doi: 10.18632/oncotarget.13760

Figure 2. Met and Prop affect the clonogenic behaviour and trigger apoptosis in breast cancer cells.

Figure 2

Cells (500 cells/well) were cultured in the presence of Met (5 mM) and/or Prop (5 μM) during 8 days. Colonies were visualized by Giemsa staining (A) in order to allow quantification (B, top panel). Clones photos were taken at different times (A, lower panel) and their size was estimated by measuring colonies diameters with the Image J software (B, bottom panel). (C, D) Analysis of the degree of apoptosis triggered by Met (5 mM) and/or Prop (5 μM): After 24 hours of treatment with the indicated drugs, 4T1 cells were collected, washed and stained with Annexin V-FITC and Propidium Iiodide. The percentage of apoptotic populations was analyzed by flow cytometry. (C) Flow cytomety profiles for 4T1 cells. (D) Quantification of the percentage of Annexin V+ apoptotic cells. (M: Met, P: Prop, M+P: Met+Prop; n = 3).