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. 2004 Dec 7;32(21):6425–6436. doi: 10.1093/nar/gkh979

Figure 1.

Figure 1

Alignment of bHLH proteins Tcfl5 and TCFL5. (A) Alignment of mouse Tcfl5 (accession no. AY234363) and human TCFL5 (accession no. AB012124). Arrows and clone names indicate the part of the protein that is encoded by the six positive one-hybrid clones. Both proteins show high-sequence conservation (83% identity), the bHLH domain (in boldface) at the C-terminus is 100% conserved. Asterisks indicate identical amino acid residues. The epitopes that were selected to generate antibodies against Tcfl5 are indicated (P3 and P4). (B) Alignment of Tcfl5, TCFL5 and other bHLH protein family members. Group B depicts the consensus bHLH amino acid sequence for this bHLH protein family (38). Amino acid residues at positions 5, 8 and 13 (in boldface) determine the family of bHLH proteins. Tcfl5 and TCFL5 follow the group B consensus at these positions. Mad and Mxi1 are the closest family members of Tcfl5 and TCFL5. Hairy and deadpan show similarity in arginine (R) content (underlined) with Tcfl5. (C) Alignment of the one-hybrid bait sequence and the putative human Calmegin promoter. The AP-2 site that is present in the mouse calmegin promoter is underlined and the 11 bp conserved element containing the non-canonical E-box CACGCG is indicated in boldface. Asterisks indicate identical nucleotides.