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. 2004 Dec 7;32(21):6425–6436. doi: 10.1093/nar/gkh979

Figure 6.

Figure 6

Gel-mobility shift assay of Tcfl5 binding to bHLH E-box oligomers. (A) Reactions in lanes 1, 3 and 5 contain the in vitro translation product of the vector pGBKT7 (control), and the reactions in lanes 2, 4 and 6 contain the in vitro translated Tcfl5 protein. The Tcfl5 protein forms a complex with both the group B bHLH canonical E-box oligomer (BE) and the hairy-related bHLH non-canonical E-box oligomer (CE), whereas no retarded band is obtained with the group A bHLH canonical E-box oligomer (AE). (B) The retarded band obtained with Tcfl5 for oligomers BE and CE is supershifted by the addition of P4-αTcfl5, in lanes 3 and 6. Reactions in lanes 1 and 4 contain the in vitro translation product of the vector pGBKT7 (control).