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. 2016 Nov 29;8(2):3144–3155. doi: 10.18632/oncotarget.13673

Figure 6. RNF2 promotes HCC cell proliferation and invasion partially through SIK1 ubiquitination.

Figure 6

A. Effects of RNF2 and SIK1 depletion on HCC Cell viability determined by MTT assays on HCC cells transfected with indicated plasmids or control(left panel). Effects of RNF2 and SIK1 depletion on HCC cell colony formation assays (right panel). B. Protein expression in HCC cells stably expressing the indicated shRNAs. C. Effects of RNF2 and SIK1 depletion on HCC tumor development. HCC cells were infected with indicated plasmids and injected subcutaneously into nude mice. Growth curve of tumor volumes. D. Estimated tumor weight for the indicated groups at day 20 after injection (left panel). (Right upper panel) representative photograph of xenograft tumors. (Right lower panel) noninvasive visualization of Luc-tagged tumor cells by whole-body fluorescence imaging showing a significant reduction in tumor size when RNF2 is depleted which was reverted by SIK1 knockdown. E. RNF2 promotes wound-healing and invasion through SIK1 signaling. Wound-healing and invasion assays for RNF2, shRNF2, and their control cells with/without SIK1 overexpression or shSIK1. F. RNF2 promotes EMT through SIK1 signaling. IF assay of E-cadherin and vimentin expressions in indicated treatment.