Table 3. Primer sequences selected for real-time RT-PCR.
| Name | Primer sequence | Distribution | AT(°C) | Product size (bp) | Extension time (s) |
|---|---|---|---|---|---|
| p53 | F: 5' - ACCTATGGAAACTACTTCCTGA - 3' R: 5' - TGGCATTCTGGGAGCTTCA - 3' |
NM_001276696.1264-403 | 60 | 139 | 39 |
| p21 | F: 5' - ACTGTCTTGTACCCTTGTGCC - 3' R: 5' - AAATCTGTCATGCTGGTCTGC - 3' |
XM_003950827572-679 | 60 | 108 | 39 |
| pP27 | F: 5' - GGCTCCGGCTAACTCTGA - 3' R: 5' - TTCTTCTGTTCTGTTGGCTCTT - 3' |
XM_5223471081-1237 | 60 | 157 | 39 |
| Cdc25C | F: 5' - ACTGGTCACCTGGATTCTT - 3' R: 5' - AAACCATTCGGAGTGCTAC - 3' |
XM_011543763.119-131 | 60 | 113 | 39 |
| Cyclin B1 | F: 5' - GTTATGCAGCACCTG - 3' R: 5' - CTTGGCTAAATCTTGAACT - 3' |
NM_0010885901388-1537 | 60 | 150 | 39 |
| Cyclin D1 | F: 5' - TGCCACAGATGTGAAGTTCATT - 3' R: 5' - CAGTCCGGGTCACACTTGAT - 3' |
NG_000002776-937 | 60 | 162 | 39 |
| Cdc2 | F: 5' - GGGCACTCCCAATAA - 3' R: 5' - GATGCTAGGCTTCCTG - 3' |
XM_572099631-723 | 60 | 93 | 39 |
| c-myc | F: 5' - AGCGACTCTGAGGAGGAACA - 3' R: 5' - TCCAGCAGAAGGTGATCCA - 3' |
XM_006761318-1425 | 60 | 108 | 39 |
| 14-3-3 | F: 5' - CAAAGACAGCACCCTCA - 3' R: 5' - TTCTGCCGCATCACAT - 3' |
XM_010379682.1845-935 | 60 | 91 | 39 |
| Bax | F: 5' - GATTGCCGCCGTGGAC - 3' R: 5' - GCCCCAGTTGAAGTTGC - 3' |
DQ926869306-393 | 60 | 88 | 39 |
| Bcl-2 | F: 5' - GCCTTCTTTGAGTTCGGTGGG - 3' R: 5' - TGTGCAGGTGCCGGTTCAG - 3' |
DQ926871938-1052 | 60 | 115 | 39 |
| MMP9 | F: 5' - TGTACCGCTATGGTTACACT - 3' R: 5' - CCTCAAAGGTTTGGAAT - 3' |
KJ897197.1211-399 | 60 | 189 | 39 |
| Nanog | F: 5' -CAAAGGCAAACAACCCACTT- 3' R: 5' -TCTGCTGGAGGCTGAGGTAT- 3' |
NM_024865.2432-589 | 60 | 158 | 39 |
| GAPDH | F: 5' - CAATGACCCCTTCATTGACC - 3' R: 5' - TGGAAGATGGTGATGGGATT - 3' |
NM_ 002046.3201-335 | 60 | 135 | 39 |
Abbreviation: AT, annealing temperature