Table 5. Primer sequences for ChIP.
| Name | Primer sequence | AT (°C) | Product size (bp) | Extension time (s) |
|---|---|---|---|---|
| p21-676 | F:5'-CCCGGAAGCATGTGACAATC-3' R:5'-CAGCACTGTTAGAATGAGCC-3' |
56 | 354 | 30 |
| p21-981 | F:5'-GGAGGCAAAAGTCCTGTGTTC-3' R:5'-GGAAGGAGGGAATTGGAGAG-3' |
56 | 306 | 30 |
| p21-2036 | F:5'-GGAGTCAGATTCTGTGTGTG-3' R:5'-CCTCTGCTTTCAGGCATTTC-3' |
56 | 368 | 30 |
| Cyclin D1-704 | F:5'-TGAAAATGAAAGAAGATGCAGTCG-3' R:5'-CTGTAGTCCGGTTTTCATAGAAATGC-3' | 57 | 328 | 30 |
| Cyclin D1-738 | F:5'-GTCCTACTTCAAATGTGTGCAGAAGG-3' R:5'-CTCCCACGAAACGCTACTTCTAGC-3' |
57 | 290 | 30 |
| Cyclin D1-744 | F:5'-CCCAGTTACTGTCGTTATCTCTCATC-3' R:5'-ATCCCTTTTGTAGCATCCCAAGAG-3' |
57 | 294 | 30 |
| c-myc-2k | F:5'-TCACGTTTGCCATTACCGGTTC -3' R:5'-TTTCAGGTTGGCTGCA G A AGGT-3' |
58 | 171 | 30 |
| c-myc-77 | F:5'-CAGGGCTTCTCAGAGGCTTGG-3' R:5'-CTGCTCGCCCGGCTCTTCC ACC-3' |
58 | 162 | 30 |
| c-myc-1572 | F:5'-CAGATCAGCAACAACCG AAA-3' R:5'-GGCCTTTTCATTGTTTTCCA-3' |
58 | 167 | 30 |
| p27 | F: 5' -CTGTCACATTCTGGAGCGTA- 3' R: 5' -AGTGGATCTTCAACTGCCTC- 3' |
60 | 230 | 30 |
| Nanog | F: 5' -GTTCTGTTGCTCGGTTTTCT- 3' R: 5' -TCCCGTCTACCAGTCTCACC- 3' |
60 | 95 | 30 |
| Poly II | F: 5' -TACTAGCGGTTTTACGGGCG- 3' R: 5' -TCGAACAGGAGGAGCAGAGAGCGA- 3' |
59 | 116 | 30 |
Abbreviation: AT, annealing temperature