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. 2016 Nov 29;8(2):3226–3236. doi: 10.18632/oncotarget.13698

Figure 4. PKCζ knockdown activates AMPK and protects human osteoblastic cells from Dex.

Figure 4

OB-6 cells were infected with lentiviral PKCζ shRNA (“-1 or -2”) or non-sense control shRNA (“scr shRNA”), and stable cells were established; miR-25-5p and PKCζ mRNA expression levels in these cells were tested A., PKCζ protein expression and AMPK activation were also tested B. Above cells were treated with or without Dex (1 μM) for 24 hours, cell viability (C, MTT assay) and apoptosis (D, Histone DNA ELISA assay) were shown. PKCζ shRNA (“-1”)-expressing OB-6 cells were transfected with miR-25, expressions of miR-25-5p E. and PKCζ mRNA F. were shown; These cells were also treated with or without Dex (1 μM) for 24 hours, cell viability G. and apoptosis H. were tested. “Ctrl” stands for untreated control group. Experiments in this figure were repeated three times, and similar results were obtained. *p<0.05 vs. “scr shRNA” cells (A, C-H).