Figure 5. AMPK activation is required for miR-25-induced cytoprotection in osteoblastic cells.

Stable OB-6 cells with miR-25 were constructed with dominant negative AMPKα (“dn-AMPKα”, T172A), AMPKα shRNA, or the scramble control shRNA (“scr shRNA”), expressions of listed proteins in these cells were tested by Western blots A. Above cells were treated with or without Dex (1 μM), cell viability (MTT assay, 24 hours, B., apoptosis intensity (Histone DNA ELISA assay, 24 hours, C. and ROS content (DCFH-DA fluorescent dye assay, 6 hours, D. were tested; NADPH activity in above cells was also shown (4 hours, E). Experiments in this figure were repeated three times, and similar results were obtained. “Ctrl” stands for untreated control group. *p<0.05 vs. “scr shRNA” cells.