Skip to main content
. 2016 Dec 10;8(2):3259–3273. doi: 10.18632/oncotarget.13887

Figure 2. Generation of stable LEF1 knockdown (LEF1 KD) RMS cell lines and expression analysis of WNT target genes.

Figure 2

(A) Representative LEF1 Western blot of human ARMS cell lines Rh41 and RMS-13 and the human ERMS cell line TE671 stably expressing a LEF1 shRNA (LEF1 KD) or empty vector control (control). HSC-70 served as loading control. Protein ladder is shown for estimation of protein size. (B) Expression of AXIN2 and c-MYC in Rh41 LEF1 KD, RMS-13 LEF1 KD and TE671 LEF1 KD are shown as fold expression to the respective control cells that were set to 1 (dashed line). Gene expression levels were normalized to 18S rRNA expression levels. Data represent mean+SEM of at least three independent experiments performed in duplicates and measured in triplicates; *P < 0.05, ***P < 0.001 by Students t-test.