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. 2016 Nov 30;8(2):3274–3288. doi: 10.18632/oncotarget.13712

Figure 1. Two-dimensional map of proteins from CLL cells and corresponding immunoblotting map.

Figure 1

Total proteins extracted from CLL cells were separated by 2-DE, stained with Coomassie Blue (A), or transferred on nitrocellulose membranes for WB and probed with CLL patient's autologous serum (B). The proteins that could be assigned to Ag spots in the WB were excised from the gel and treated with trypsin. The resulting fragments were analyzed by MS. The location of proteins recognized by a representative CLL serum (patient 29) are indicated by boxes in the proteomic map and in the corresponding WB membrane.