Table 1. Proteins recognized by at least 3 CLL sera.
| MW (kDa)/pI | Swiss-Prot Accession number | Protein name | Sequence coverage (%) | Metched peptides | Mascot score | Recurrence of antibodies (%) | ||
|---|---|---|---|---|---|---|---|---|
| CLL patients | Healthy Donors | p | ||||||
| 47,481/7.10 | Q6GMP2 | α-Enolase (ENO1) | 53 | 20 | 250 | 19 (54) | 0 | 0.006 |
| 33,073/5.45 | P52907 | F-actin-capping protein subunit alpha-1 (CAZA1) | 32 | 6 | 73 | 9 (26) | 0 | ns |
| 36,201/8.57 | P04406 | Glyceraldehyde-3-phosphate dehydrogenase (G3P) | 33 | 10 | 125 | 9 (26) | 0 | ns |
| 39,550/6.20 | P11177 | Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (ODPB) | 43 | 11 | 93 | 8 (23) | 0 | ns |
| 23,031/5.10 | P52566 | Rho GDP-dissociation inhibitor 2 (GDIR2) | 56 | 9 | 93 | 7 (20) | 0 | ns |
| 37,462/8.97 | P22626 | Heterogeneous nuclear ribonucleoproteins A2/B1 (HNRNPA2B1) | 39 | 13 | 151 | 6 (17) | 0 | ns |
| 53,676/5.06 | P08670 | Vimentin (VIME) | 53 | 20 | 250 | 6 (17) | 1 (13) | ns |
| 42,052/5.29 42,108/5.31 | Q96HG5 P63261 | Actin, cytoplasmic 1 (ACTB) - Actin, cytoplasmic 2 (ACTG) | 39 | 13 | 145 | 5 (14) | 0 | ns |
| 31,616/5.36 | P47756 | F-actin-capping protein subunit beta (CAPZB) | 36 | 8 | 98 | 5 (14) | 0 | ns |
| 45,753/6.86 | P14902 | Indoleamine 2,3-dioxygenase 1 (I23O1) | 23 | 8 | 66 | 5 (14) | 0 | ns |
| 29,843/5.57 | P35232 | Prohibitin (PHB) | 51 | 10 | 148 | 5 (14) | 0 | ns |
| 61,187/5,70 | P10809 | 60 kDa heat shock protein (HSP60) | 37 | 14 | 154 | 4 (11) | 1 (13) | ns |
| 39,851/8.30 | P04075 | Fructose-bisphosphate aldolase A (ALDOA) | 44 | 13 | 106 | 4 (11) | 1 (13) | ns |
| 23,569/5.43 | P09211 | Glutathione S-transferase P (GSTP1) | 55 | 7 | 96 | 4 (11) | 0 | ns |
| 71,082/8.52 | P11142 | Heat shock cognate 71 kDa protein (HSP7C) | 37 | 16 | 197 | 3 (9) | 0 | ns |
| 49,240/6.03 | Q8NFZ5 | TNFAIP3-interacting protein 2 (TNIP2) | 17 | 8 | 69 | 3 (9) | 0 | ns |
| 51,230/5.39 | P61978 | Heterogeneous nuclear ribonucleoprotein K (HNRNPK) | 34 | 16 | 109 | 3 (9) | 0 | ns |
| 64,720/8.46 | P14866 | Heterogeneous nuclear ribonucleoprotein L (HNRPL) | 16 | 8 | 78 | 3 (9) | 0 | ns |
MW, Molecular weight; pI, isoelectrical point. Sequence coverage (%) is the percentage of the protein primary sequence covered by peptides obtained by tripsyn digestion of the Ag spot. Matched peptides is the rate between the number of peptides corresponding to the primary sequence of the protein and the total number of peptides analyzed. Mascot score is a measure of the correspondence of peptides to the protein primary sequence.