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. 2016 Dec 10;8(2):3495–3508. doi: 10.18632/oncotarget.13849

Figure 1. Kym-1 cells, but not other RMS cell lines, are sensitive to LCL161 as a single agent.

Figure 1

A. Alamar Blue viability assays of human RMS cell lines (Kym-1, RH36, RH41, RD, RH30, RH28, RH18) and the murine cell line 76-9 with increasing concentration of LCL161 for 24 h. B. Human RMS cell lines were analysed by western blot for cIAP1 and tubulin protein expression following treatment with vehicle control (DMSO) or 5 μM LCL161 (10 nM LCL161 was used for Kym-1 cells) for 24 h. The mouse C2C12 myoblast, 76-9 RMS, and 1863 sarcoma cell lines were also analysed by western blot for cIAP1and tubulin protein expression following treatment with DMSO or 5 μM LCL161 for 24 h. C, D. Endogenous protein expression of apoptosis pathway effectors and immune modulator receptors was analysed by western blot in human RMS cell lines (C and D, respectively).