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Analysis of CpG methylation in 10 clones (A–J) each derived from two bisulfite-converted colon tumor DNA samples (T1 and T2) and two normal colon DNA samples (N1 and N2), respectively, by RNA cleavage and MALDI-TOF (left) and sequencing (right). CpG methylated (black circle); CpG unmethylated (white circle); no fragment indicative of CpG methylation observed or ambiguous sequence (gray circle); CpG not accessible for analysis (cross).
