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. 2004 Dec 2;32(21):e171. doi: 10.1093/nar/gnh170

Figure 4.

Figure 4

Analysis of human H1 promoter-mediated siRNA silencing of GUS gene expression in transgenic tobacco. (A) GUS protein activity in leaves of the control plants (C) and 10 pGPH1-HPT-GT2 transgenic lines. Mean values were calculated from three independent measurements per line. (B) Loading control for gel blot analysis showing 25S rRNA transcript levels. (C) The same gel blot as in (B) was used to characterize the GUS mRNA level with a GUS cDNA probe. (D) Gel blot detection of small RNAs of ∼21 nt, as indicated, using a GUS cDNA probe. RNA was isolated from a portion of the leaves used for GUS protein activity assay in (A).