Fig 2. Levels of expression of decoys and endogenous AR in vivo.
A, Western blot analysis for GFP with a representative animal showing extremely high expression of GFP delivered by lentivirus to the xenograft, yet non-detectable levels of expression in the spleen, liver, lung, heart, and kidney of the same animal. Similar levels of protein (40μg) from whole cell lysates. The membrane was stripped and re-probed for β-actin as a loading control. B, AR, AR1-558 (ARN), GFP, and GFP-AR1-558 (GFP-ARN) protein levels in harvested xenografts. A non-specific diffuse band migrates slightly slower than AR1-558 and was most apparent in the mock-treated lysates.