Fig 4. Decoys block the expression of androgen-regulated genes.
Real-time qPCR was performed using total RNA isolated from: A, LNCaP cells stably transfected with vector (Vec) or decoys (ARN) and treated for 24 hours with 10nM R1881; or B, xenografts injected with mock, AR1-558 (ARN), GFP, and GFP-AR1-558 (GFP-ARN). Transcript levels of HMGCR, KLK2, KLK3/PSA, MAF, RHOU, and SAT, were normalized to levels of GAPDH. The ratio of each transcript to GAPDH is plotted as fold-change. The bars represent the mean ± SD (n = 3). Student t-test: * p<0.05; **: p<0.01.