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. 2017 Mar 17;12(3):e0174134. doi: 10.1371/journal.pone.0174134

Fig 5. Decoys block AR-ARE interaction.

Fig 5

ChIP was performed in non-transfected and stably transfected (vector or decoy) cells in response to androgen. Parental LNCaP cells (untransfected) or stables expressing vector or decoy were treated ± androgens (R1881, 10 nM) for 3 hours and used in ChIP analyses with rabbit IgG (no antibody negative control) and anti-AR (mAb; C19 antibody to AR LBD). Eluted DNA fragments were purified and used for qPCR with primers designed to amplify the PSA ARE. Bars show the percentage input as the mean ± SD (n = 3). A representative result from repeated experiments is shown.