Table 2. Phosphorylation site mutations in Smo.

Allele name	Mutated region(s)*	Region
All PKA Ala	KKGAYFH	PKA
	RKNALDS
	RRNAVDS
	RREASTS
	KKTAYPN
	KRRAANA
I Ala	LYNAHADPV	I
II Ala	DLNAAEANDIASTW	II
III Ala	TGNAAAHGP	III
IV Ala	RKNALDAEIAVSV	IV
V Ala	RHVAVEARRNAVDAQVAVKI	V
VI Ala	RREAAAAVEAQVI	VI
VII Ala	QDMAAAAEED	VII
VIII Ala	QEIAEDD	VIII
680 Ala	RHVAVESRRNSVDSQVSVKI	V
687 Ala	RHVSVESRRNAVDSQVSVKI	V
680,687 Ala	RHVAVESRRNAVDSQVSVKI	V
740 Ala	RREASTSVESQVI	VI
687,740 Ala	RHVSVESRRNAVDSQVSVKI	V
	RREASTSVESQVI	VI
687,740 Glu + other V,VI Ala	RHVAVEARRNEVDAQVAVKI	V
	RREEAAAVEAQVI	VI
V Glu+740 Ala	RHVEVEERRNEVDEQVEVKI	V
	RREASTSVESQVI	VI
V Glu+740 Glu	RHVEVEERRNEVDEQVEVKI	V
	RREESTSVESQVI	VI

Shown in most cases are the Ala variants created to mutate a given region or individual phosphoresidue to alanine. The corresponding Glu variants (not shown) change the underlined residues to glutamic acid instead. The following alleles are simply combinations of the alleles listed above, encoding variants with phospho-residues in two or more regions mutated all to Ala or all to Glu: I,II Ala; I,II Glu; III,IV Ala; III,IV Glu; I,II,III,IV Ala; I,II,III,IV Glu; III,V,VI Ala; III,V,VI Glu; III,V,VI,VIII Ala; III,V,VI,VIII Glu; all PKA+ III,V,VI,VIII Ala; all PKA+III,V,VI,VIII Glu; V,VI Glu; V,VI,VIII Glu.

^*Mutated residues are underlined.