Fig. 6.

DnaK NBD mutants defective in Hsp90_Ec interaction are defective in functional collaboration with Hsp90_Ec in luciferase reactivation. Heat-inactivated luciferase was reactivated as described in Materials and Methods using DnaK wild-type or mutant, CbpA, GrpE and Hsp90_Ec as indicated. (a-l) Luciferase reactivation by DnaK wild-type (black/gray) or mutant (color), as indicated in panels a-l in combination with CbpA and GrpE (open symbols and dashed lines) or CbpA, GrpE and Hsp90_Ec (filled symbols and solid lines) as a function of time. Dotted lines indicate luciferase alone control. In a-l, data from three or more replicates are presented as mean ± SEM. For some points, the symbols obscure the error bars.